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EasyStep ELISA
source:ELK Biotechnologydate:2024-03-06views:486

EasyStep ELISA is a new developed method by ELK Biotechnology.

It is based on a three-step method and directly labels the detection antibody with HRP, achieving one-step sampling. This method not only saves time, but also improves experimental efficiency. ELK's unique standard strips can also increase the number of sample tests and facilitate scientific research.

 

 

Easy Step principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The plateprovided in this kit has been pre-coated with an specific antibody , then, add standard/samples. Proteins in the standards or proteins in the sample bind to the antibodies on the microplate. Next, add HRP-conjugated antibody, wash the unbound HRP-conjugated antibody, and then add TMB substrate for color development. TMB is converted into blue under the catalysis of peroxidase and into yellow under the action of acid. The color  depth is positively related to the concentration of the target protein. Finally, use a microplate reader to measure the absorbance (OD value) at a wavelength of 450 nm, and calculate the sample concentration by drawing a standard curve.

 

 

 

 

Easy Step Advantages:

More samples

◆  96 samples/96T        ◆  48 samples/48T

 

High speed

1.5h assay time only, making your scientific research one step faster.


Easy operation

all raw materials have been tested for thermal stability..No standard dilution step, which can reduce operating errors and improve the accuracy of the experiment.

 

Less sampling

50ul sampling, more tests can be completed with limited samples.

 

Worry-free

Provide technical guidance throughout the entire process.

 

 

One-Step VS three-steps

 

Easy Step Catalog:

Cat.No.  Name Detection range  Sensitivity
ELK001ES EasyStep Human IL6(Interleukin 6) ELISA Kit 1.56-100 pg/mL 0.1pg/ml
ELK002ES EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit 0.16ng/ml-10ng/ml 7.5 pg/ml
ELK003ES EasyStep Human PCT(Procalcitonin) ELISA Kit 7.81 pg/ml-500 pg/ml 1.5 pg/ml
ELK004ES EasyStep Human AMH(Anti-Mullerian Hormone) ELISA Kit 0.31 ng/ml-20 ng/ml 0.028 ng/ml
ELK005ES EasyStep Human Cys-C(CystatinC) ELISA Kit 78.1 pg/ml-5000 pg/ml 25 pg/ml
ELK006ES EasyStep Human GH(Growth Hormone) ELISA Kit 12.5 pg/ml-800pg/ml 0.2pg/ml
ELK007ES EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit 31.25 pg/ml-2000 pg/ml 6.8 pg/ml
ELK008ES EasyStep Human sST2(Soluble Suppression Of Tumorigenicity 2) ELISA Kit 0.47 ng/ml-30 ng/ml 0.25 ng/ml
ELK009ES EasyStep Human OC(Osteocalcin) ELISA Kit 0.23 ng/ml-15 ng/ml 0.14 ng/ml
ELK010ES EasyStep Human PGII(Pepsinogen II) ELISA Kit 0.16 ng/ml-10 ng/ml 7 pg/ml
ELK011ES EasyStep Human PSA(ProstateSpecific Antigen) ELISA Kit 0.31 ng/ml-20 ng/ml 80 pg/ml
ELK012ES EasyStep Human CRP(C Reactive Protein) ELISA Kit 0.23 ng/mL - 15ng/mL 53 pg/mL
ELK013ES EasyStep Human PRL(Prolactin) ELISA Kit 0.313 ng/mL- 20 ng/mL 248 pg/mL
ELK014ES EasyStep Human HE4(Epididymal protein 4) ELISA kit 0.16 ng/mL- 10 ng/mL 160 pg/mL
ELK015ES EasyStep Human HBP(Heparin-Binding Protein)ELISA kit 39 pg/mL - 2500 pg/mL 39 pg/mL
ELK016ES EasyStep Human CP(C-Peptide) ELISA Kit 0.625 ng/mL - 40 ng/mL 237 pg/mL
ELK017ES EasyStep Human FE(Ferritin) ELISA Kit 0.313 ng/mL - 20 ng/mL 3.2 pg/mL
ELK018ES EasyStep Mouse FE(Ferritin) ELISA Kit 0.625 ng/mL - 40 ng/mL 6.4 pg/mL
ELK019ES EasyStep Human MPO(Myeloperoxidase) ELISA Kit 0.625 ng/mL - 40 ng/mL 184 pg/mL
ELK020ES EasyStep Human E2(Estradiol) ELISA Kit 25 pg/mL - 1600 pg/mL 25 pg/mL
ELK021ES EasyStep Mouse E2(Estradiol) ELISA Kit 25 pg/mL - 1600 pg/mL 25 pg/mL
ELK022ES EasyStep Rat E2(Estradiol) ELISA Kit 25 pg/mL - 1600 pg/mL 25 pg/mL
ELK023ES EasyStep Human ANXA1(Annexin A1) ELISA Kit 0.313 ng/mL - 20 ng/mL 16.8 pg/ml
ELK024ES EasyStep Human EFNA1(Ephrin A1) ELISA Kit 0.16ng/ml-10ng/ml 22.5 pg/ml
ELK025ES EasyStep Human VASP(Vasodilator Stimulated Phosphoprotein) ELISA Kit 0.16ng/ml-10ng/ml 17.5 pg/ml
ELK026ES EasyStep Human CEACAM1(Carcinoembryonic Antigen Related Cell Adhesion Molecule 1) ELISA Kit 1.57-100 ng/mL 89.5 pg/ml
ELK027ES EasyStep Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit 31.25 pg/mL - 2000 pg/mL 15 pg/ml
ELK028ES EasyStep Human ADP(Adiponectin) ELISA Kit 3.13 ng/ml - 200 ng/ml 1.9 ng/ml
ELK029ES EasyStep Mouse ADP(Adiponectin) ELISA Kit 7.81 ng/ml - 500 ng/ml 4.75 ng/ml
ELK030ES EasyStep Rat ADP(Adiponectin) ELISA Kit 7.81 ng/ml - 500 ng/ml 4.75 ng/ml
ELK031ES EasyStep Human FSH(Follicle Stimulating Hormone) ELISA Kit 1.56 mIU/ml-100 mIU/ml 0.016 mIU/ml
ELK032ES EasyStep Human MIg(Monokine Induced By Interferon Gamma) ELISA Kit 78.1 pg/ml-5000 pg/ml 15.7 pg/ml
ELK033ES EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit 78.1 pg/ml-5000 pg/ml 31 pg/ml
ELK034ES EasyStep Human OPG(Osteoprotegerin) ELISA Kit 31.25 pg/mL - 2000 pg/mL 23.6 pg/ml
ELK035ES EasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit 0.94 ng/ml - 60 ng/ml 0.94 ng/ml
ELK036ES EasyStep Human PSMA(Prostate specific membrane antigen) ELISA Kit 0.625 ng/mL - 40 ng/mL 0.165 ng/ml
ELK037ES EasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit 78.1 pg/ml-5000 pg/ml 21 pg/mL
ELK038ES EasyStep Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit 15.63-1000 pg/mL 5.6 pg/ml
ELK039ES EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit 0.16 ng/ml-10 ng/ml 37 pg/mL
ELK040ES EasyStep Human LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit 0.78 ng/ml - 50 ng/ml 0.78 ng/ml
ELK041ES EasyStep Human MMP9(Matrix Metalloproteinase 9) ELISA Kit 1.25 ng/mL - 80 ng/mL 1.25 ng/mL
ELK042ES EasyStep Human SAA(Serum Amyloid A) ELISA Kit 0.78 ng/mL - 50 ng/mL 0.15 ng/mL
ELK043ES EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit 1.25 ng/mL - 80 ng/mL 0.87 ng/mL
ELK044ES EasyStep Human GDF15(Growth Differentiation Factor 15) ELISA Kit 46.88 pg/mL - 3000 pg/mL 5.22 pg/mL
ELK045ES EasyStep Human  Mouse PG(Progesterone) ELISA Kit 1.56 ng/ml - 100 ng/ml 0.7 ng/ml
ELK046ES EasyStep Mouse PG(Progesterone) ELISA Kit 1.56 ng/ml - 100 ng/ml 0.7 ng/ml
ELK047ES EasyStep Rat PG(Progesterone) ELISA Kit 1.56 ng/ml - 100 ng/ml 0.7 ng/ml
ELK048ES EasyStep Human fT3(Free Triiodothyronine) ELISA Kit 0.31 ng/ml - 20 ng/ml 0.31 ng/ml
ELK049ES EasyStep Mouse fT3(Free Triiodothyronine) ELISA Kit 0.31 ng/ml - 20 ng/ml 0.31 ng/ml
ELK050ES EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit 0.31 ng/ml - 20 ng/ml 0.31 ng/ml
ELK051ES EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit 78.13 ng/ml - 5000 ng/ml 7.7 ng/ml
ELK052ES EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit 78.13 ng/ml - 5000 ng/ml 7.7 ng/ml
ELK053ES EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit 78.13 ng/ml - 5000 ng/ml 7.7 ng/ml
ELK054ES EasyStep Human T4(Thyroxine) ELISA Kit 9.38 ng/ml - 600 ng/ml 7 ng/ml
ELK055ES EasyStep Mouse T4(Thyroxine) ELISA Kit 9.38 ng/ml - 600 ng/ml 7 ng/ml
ELK056ES EasyStep Rat T4(Thyroxine) ELISA Kit 9.38 ng/ml - 600 ng/ml 7 ng/ml
ELK057ES EasyStep Human cTnI ( Cardiac Troponin I) ELISA Kit 0.39 ng/ml - 25 ng/ml 19.6 pg/ml
ELK058ES EasyStep Human Lpa(Lipoprotein, a) ELISA Kit 0.78 ng/ml - 50 ng/ml 0.38 ng/ml
ELK059ES EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit 78.13 pg/ml - 5000 pg/ml 0.98 pg/ml
ELK060ES EasyStep Human RBP(Retinol Binding Protein) ELISA Kit 1.56 ng/ml - 100 ng/ml 0.34 ng/ml
ELK061ES EasyStep Human PGI(Pepsinogen I) ELISA Kit 0.31 ng/ml - 20 ng/ml 18 pg/ml
ELK062ES EasyStep Human IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit 3.13 ng/ml - 200 ng/ml 1.4 ng/ml
ELK063ES EasyStep Mouse IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit 15.63 ng/ml - 1000 ng/ml 7 ng/ml
ELK064ES EasyStep Rat IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit 15.63 ng/ml - 1000 ng/ml 7 ng/ml