MRP-L22 rabbit pAb

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MRP-L22 rabbit pAb

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ES5671

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	MRP-L22 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	MRPL22; MRPL25; RPML25; HSPC158; 39S ribosomal protein L22; mitochondrial; L22mt; MRP-L22; 39S ribosomal protein L25, mitochondrial; L25mt; MRP-L25
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human MRPL22. AA range:91-140
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	24kD
GeneID:	29093
Human Swiss-Prot No:	Q9NWU5
Cellular localization:	Mitochondrion .
Background:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provide

Western blot analysis of lysates from HepG2 cells, using MRPL22 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from Jurkat cells using MRPL22 antibody.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).