EphB4 rabbit pAb

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EphB4 rabbit pAb

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ES5136

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	EphB4 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	EPHB4; HTK; MYK1; TYRO11; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human EPHB4. AA range:571-620
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	108kD
GeneID:	2050
Human Swiss-Prot No:	P54760
Cellular localization:	Cell membrane ; Single-pass type I membrane protein .
Background:	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008],

Western Blot analysis of HY926 cells using EphB4 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from Jurkat and 293 cells, using EPHB4 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).