POLR3H rabbit pAb

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POLR3H rabbit pAb

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ES5004

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	POLR3H rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human RPC8. AA range:151-200
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	23kD
GeneID:	171568
Human Swiss-Prot No:	Q9Y535
Cellular localization:	Nucleus.
Background:	function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.,similarity:Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.,

Western Blot analysis of K562 cells using POLR3H Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i
Western blot analysis of lysates from K562 cells, using RPC8 Antibody. The lane on the right is blocked with the synthesized peptide.