ME3 rabbit pAb

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ME3 rabbit pAb

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ES4565

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	ME3 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	ME3; NADP-dependent malic enzyme; mitochondrial; NADP-ME; Malic enzyme 3
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human ME3. AA range:545-594
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	67kD
GeneID:	10873
Human Swiss-Prot No:	Q16798
Cellular localization:	Mitochondrion matrix.
Background:	Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

Western Blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i
Western blot analysis of lysates from RAW264.7 and NIH/3T3 cells, using ME3 Antibody. The lane on the right is blocked with the synthesized peptide.