NUDC rabbit pAb

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NUDC rabbit pAb

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ES4543

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	NUDC rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	NUDC; Nuclear migration protein nudC; Nuclear distribution protein C homolog
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human NudC. AA range:282-331
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	38kD
GeneID:	10726
Human Swiss-Prot No:	Q9Y266
Cellular localization:	Cytoplasm, cytoskeleton. Nucleus . Cytoplasm, cytoskeleton, spindle . Midbody . In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. Localizes to the mitotic spindle in a EML4-dependent manner (PubMed:25789526). .
Background:	This gene encodes a nuclear distribution protein that plays an essential role in mitosis and cytokinesis. The encoded protein is involved in spindle formation during mitosis and in microtubule organization during cytokinesis. Pseudogenes of this gene are found on chromosome 2. [provided by RefSeq, Feb 2012],

Western Blot analysis of rat cells using NUDC Polyclonal Antibody diluted at 1:2000
Western Blot analysis of AD293 using NUDC Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from rat brain cells, using NudC Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).