MLC-2 (phospho Thr17/S18) rabbit pAb

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MLC-2 (phospho Thr17/S18) rabbit pAb

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ES4525

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	MLC-2 (phospho Thr17/S18) rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	MYL12A; MLCB; MRLC3; RLC; Myosin regulatory light chain 12A; MLC-2B; Myosin RLC; Myosin regulatory light chain 2; nonsarcomeric; Myosin regulatory light chain MRLC3; MYL12B; MRLC2; MYLC2B; Myosin regulatory light chain 12B; MLC-2A; MLC-2; M
Source:	Rabbit
Dilutions:	WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18. AA range:5-54
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Molecular Weight:	20kD
GeneID:	10398/10627
Human Swiss-Prot No:	P19105/O14950/P24844
Cellular localization:	stress fiber,cytosol,brush border,myosin complex,myosin II complex,Z disc,apical part of cell,extracellular exosome,
Background:	This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014],

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MLC-2 (Phospho-Thr17+Ser18) Antibody
Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).