Hec1 rabbit pAb

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Hec1 rabbit pAb

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ES4468

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Hec1 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	NDC80; HEC; HEC1; KNTC2; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human KNTC2. AA range:351-400
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	73kD
GeneID:	10403
Human Swiss-Prot No:	O14777
Cellular localization:	Nucleus . Chromosome, centromere, kinetochore . Localizes to kinetochores from late prophase to anaphase (PubMed:14699129). Localizes specifically to the outer plate of the kinetochore (PubMed:14699129). .
Background:	This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011],

Immunofluorescence analysis of Hela cell. 1,Hec1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Kif 7 Monoclonal Antibody(3F8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:100
Western Blot analysis of Jurkat cells using Hec1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunofluorescence analysis of HUVEC cells, using KNTC2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using KNTC2 Antibody. The lane on the right is blocked with the synthesized peptide.