MPP10 rabbit pAb

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MPP10 rabbit pAb

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ES3809

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	MPP10 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	MPHOSPH10; MPP10; U3 small nucleolar ribonucleoprotein protein MPP10; M phase phosphoprotein 10
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	Synthesized peptide derived from MPP10 . at AA range: 60-140
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	78kD
GeneID:	10199
Human Swiss-Prot No:	O00566
Cellular localization:	Nucleus, nucleolus . Chromosome . Fibrillar region of the nucleolus (PubMed:9450966). After dissolution of the nucleolus in early M phase becomes associated with chromosomes through metaphase and anaphase (PubMed:9450966). In telophase localized to small cellular prenucleolar bodies that not always contain fibrillarin (PubMed:9450966). The reassociation with nucleolus is preceeded by the arrival of fibrillarin (PubMed:9450966). .
Background:	This gene encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing. [provided by RefSeq, Dec 2010],

Western Blot analysis of extracts from Jurkat cells, using MPP10 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).