TLE4 rabbit pAb

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TLE4 rabbit pAb

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ES3615

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	TLE4 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	TLE4; KIAA1261; Transducin-like enhancer protein 4
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human TLE4. AA range:161-210
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	83kD
GeneID:	7091
Human Swiss-Prot No:	Q04727
Cellular localization:	Nucleus.
Background:	caution:Product of a dubious CDS prediction. Found in the 3'UTR of TLE4. No homolog. Large scale identification of a phosphorylated peptide (PubMed:15302935) would require further confirmation.,function:Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.,similarity:Belongs to the WD repeat Groucho/TLE family.,similarity:Contains 7 WD repeats.,subunit:Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2.,tissue specificity:In all tissues examined, mostly in brain, and muscle.,

Western Blot analysis of various cells using TLE4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from Jurkat, 293, and HUVEC cells, using TLE4 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).