Tetraspanin-8 rabbit pAb

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Tetraspanin-8 rabbit pAb

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ES3583

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Tetraspanin-8 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	TSPAN8; TM4SF3; Tetraspanin-8; Tspan-8; Transmembrane 4 superfamily member 3; Tumor-associated antigen CO-029
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human TSPAN8. AA range:96-145
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	26kD
GeneID:	7103
Human Swiss-Prot No:	P19075
Cellular localization:	Membrane; Multi-pass membrane protein.
Background:	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using Tetraspanin-8 Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of HeLa cells, using TSPAN8 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 and COLO cells, using TSPAN8 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).