ST18 rabbit pAb

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ST18 rabbit pAb

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ES3504

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	ST18 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	ST18; KIAA0535; ZNF387; Suppression of tumorigenicity 18 protein; Zinc finger protein 387
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human ZNF387. AA range:41-90
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	115kD
GeneID:	9705
Human Swiss-Prot No:	O60284
Cellular localization:	Nucleus .
Background:	function:Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.,similarity:Contains 6 C2HC-type zinc fingers.,tissue specificity:Detected at low levels in heart, liver, kidney, skeletal muscel, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells.,

Western Blot analysis of various cells using ST18 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HUVEC cells, using ZNF387 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from COLO205 cells using ZNF387 antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).