Siah-2 rabbit pAb

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Siah-2 rabbit pAb

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ES3443

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Siah-2 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human SIAH2. AA range:241-290
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	38kD
GeneID:	6478
Human Swiss-Prot No:	O43255
Cellular localization:	Cytoplasm . Nucleus . Predominantly cytoplasmic. Partially nuclear. .
Background:	This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using Siah-2 Polyclonal Antibody
Western blot analysis of lysates from COLO cells, using SIAH2 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).