Rab 35 rabbit pAb

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Rab 35 rabbit pAb

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ES3291

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Rab 35 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	RAB35; RAB1C; RAY; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	24kD
GeneID:	11021
Human Swiss-Prot No:	Q15286
Cellular localization:	Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). .
Background:	function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,

Western Blot analysis of various cells using Rab 35 Polyclonal Antibody
Western blot analysis of RAB35 Antibody. The lane on the right is blocked with the RAB35 peptide.
Western blot analysis of the lysates from HT-29 cells using RAB35 antibody.
Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).