PRIM1 rabbit pAb

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PRIM1 rabbit pAb

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ES3260

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	PRIM1 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	PRIM1; DNA primase small subunit; DNA primase 49 kDa subunit; p49
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human PRIM1. AA range:371-420
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	50kD
GeneID:	5557
Human Swiss-Prot No:	P49642
Cellular localization:	nucleoplasm,alpha DNA polymerase:primase complex,membrane,primosome complex,
Background:	The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using PRIM1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from COLO and HeLa cells, using PRIM1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from K562 cells using PRIM1 antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).