PDK2 rabbit pAb

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PDK2 rabbit pAb

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ES3170

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	PDK2 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	PDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human PDK2. AA range:260-309
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	46kD
GeneID:	5164
Human Swiss-Prot No:	Q15119
Cellular localization:	Mitochondrion matrix.
Background:	This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010],

Western Blot analysis of various cells using PDK2 Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from HepG2 cells, using PDK2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using PDK2 antibody.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).