Pdcd-4 rabbit pAb

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Pdcd-4 rabbit pAb

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ES3161

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Pdcd-4 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	PDCD4; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human PDCD4. AA range:420-469
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	51kD
GeneID:	27250
Human Swiss-Prot No:	Q53EL6
Cellular localization:	Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133). .
Background:	This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010],

Western Blot analysis of various cells using Pdcd-4 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i
Immunofluorescence analysis of HeLa cells, using PDCD4 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using PDCD4 Antibody. The lane on the right is blocked with the synthesized peptide.