Nanog P8 rabbit pAb

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Nanog P8 rabbit pAb

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ES2895

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Nanog P8 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	NANOGP8; Putative homeobox protein NANOGP8
Source:	Rabbit
Dilutions:	WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200
Immunogen:	The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	35kD
GeneID:	388112
Human Swiss-Prot No:	Q6NSW7
Cellular localization:	Nucleus .
Background:	This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq, Sep 2009],

Western Blot analysis of various cells using Nanog P8 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from HUVEC, HT-29, HepG2, and Jurkat cells, using NANOGP8 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).