N/H/K-Ras rabbit pAb

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N/H/K-Ras rabbit pAb

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ES2886

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	N/H/K-Ras rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human RASH/RASK. AA range:1-50
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	21kD
GeneID:	3265/3845/4893
Human Swiss-Prot No:	P01111/P01112/P01116
Cellular localization:	Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor . Shuttles between the plasma membrane and the Golgi apparatus. .
Background:	This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011],

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of various cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000
Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RASH/RASK Antibody. The picture on the right is blocked with the synthesized peptide.