MRP-L12 rabbit pAb

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MRP-L12 rabbit pAb

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ES2828

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	MRP-L12 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	MRPL12; RPML12; 39S ribosomal protein L12; mitochondrial; L12mt; MRP-L12; 5c5-2
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	The antiserum was produced against synthesized peptide derived from human MRPL12. AA range:31-80
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	21kD
GeneID:	6182
Human Swiss-Prot No:	P52815
Cellular localization:	Mitochondrion .
Background:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using MRP-L12 Polyclonal Antibody
Western blot analysis of lysates from COLO cells, using MRPL12 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).