Histone 1.0 rabbit pAb

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Histone 1.0 rabbit pAb

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ES2517

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Histone 1.0 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	H1F0; H1FV; Histone H1.0; Histone H1'; Histone H1(0)
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Histone 1F0. AA range:71-120
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	28kD
GeneID:	3005
Human Swiss-Prot No:	P07305
Cellular localization:	Nucleus . Chromosome . The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
Background:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015],

Western Blot analysis of various cells using Histone 1.0 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from A549 cells, using Histone 1F0 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).