FN1 rabbit pAb

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FN1 rabbit pAb

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ES2355

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	FN1 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Fibronectin 1. AA range:2337-2386
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	260kD
GeneID:	2335
Human Swiss-Prot No:	P02751
Cellular localization:	Secreted, extracellular space, extracellular matrix .
Background:	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016],

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western blot analysis of lysates from 1) 3T3 , 2) KB ,3) K562 ,4) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) (cat:YM302
Western Blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1:2000
Western Blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1:2000