ETAR rabbit pAb

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ETAR rabbit pAb

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ES2305

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	ETAR rabbit pAb
Reactivity:	Human;Mouse;Monkey
Alternative Names:	EDNRA; ETA; ETRA; Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human EDNRA. AA range:378-427
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	48kD
GeneID:	1909
Human Swiss-Prot No:	P25101
Cellular localization:	Cell membrane; Multi-pass membrane protein.
Background:	This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

Western Blot analysis of various cells using ETAR Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunofluorescence analysis of LOVO cells, using EDNRA Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, using EDNRA Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).