Cytochrome b5 rabbit pAb

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Cytochrome b5 rabbit pAb

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ES2121

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Cytochrome b5 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	15kD
GeneID:	1528
Human Swiss-Prot No:	P00167
Cellular localization:	[Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm.
Background:	The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],

Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells, using CYB5 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from A549 cells, using CYB5 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).