CstF-64 rabbit pAb

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CstF-64 rabbit pAb

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ES2067

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	CstF-64 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	CSTF2; Cleavage stimulation factor subunit 2; CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CstF-64
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human CSTF2. AA range:11-60
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	60kD
GeneID:	1478
Human Swiss-Prot No:	P33240
Cellular localization:	Nucleus . Localized with DDX1 in cleavage bodies.
Background:	This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using CstF-64 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunofluorescence analysis of HeLa cells, using CSTF2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293, K562, and Jurkat cells, using CSTF2 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).