Casein Kinase IIα rabbit pAb

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Casein Kinase IIα rabbit pAb

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ES1845

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Casein Kinase IIα rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	CSNK2A1; CK2A1; Casein kinase II subunit alpha; CK II alpha
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Casein Kinase II alpha. AA range:221-270
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	45kD
GeneID:	1457
Human Swiss-Prot No:	P68400
Cellular localization:	Nucleus .
Background:	Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2014],

Western Blot analysis of various cells using Casein Kinase IIα Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HeLa and Jurkat cells, using Casein Kinase II alpha Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from 293 cells using Casein Kinase II α antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).