CARD 10 rabbit pAb

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CARD 10 rabbit pAb

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ES1839

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	CARD 10 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	120kD
GeneID:	29775
Human Swiss-Prot No:	Q9BWT7
Cellular localization:	Cytoplasm .
Background:	The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008],

Western Blot analysis of various cells using CARD 10 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells, using CARD10 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa and 293 cells, using CARD10 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).