Iba1 rabbit pAb

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Iba1 rabbit pAb

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ES11111

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Iba1 rabbit pAb
Reactivity:	Human;Rat;Mouse
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	Synthesized peptide derived from part region of human protein
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	16kD
GeneID:	199
Human Swiss-Prot No:	P55008
Cellular localization:	Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, phagocytic cup . Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. .
Background:	This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016],

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night