Caspase 9 Mouse mAb

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Caspase 9 Mouse mAb

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EM1077

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Caspase 9 Mouse mAb
Reactivity:	Human, Rat, Mouse
Alternative Names:	APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody
Source:	Mouse
Dilutions:	WB 1:1,000-5,000 IP 1:200 IF 1:100-200
Immunogen:	Synthetic Peptide
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/mL
Observed Band:	47（51）kDa
GeneID:	842
Human Swiss-Prot No:	P55211
Cellular localization:	Cytosol,Mitochondrion,Nucleus
Background:	Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1）1:2,000 2）1:5,000
Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100.