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Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
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ELK9972
Size: Price:
48 T $320.00
96 T $458.00
96 T*5 $1947.00

Overview

Product name: Horse NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
Reactivity: Horse
Alternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Uniprot ID: F6TME6
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse NFKB-p105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse NFKB-p105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse NFKB-p105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse NFKB-p105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.179 2.097
5.00 1.663 1.581
2.50 1.185 1.103
1.25 0.876 0.794
0.63 0.487 0.405
0.32 0.264 0.182
0.16 0.186 0.104
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NFKB-p105 and the recovery rates were calculated by comparing the measured value to the expected amount of NFKB-p105 in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 78-96% 87%
Heparin plasma(n=5) 87-101% 94%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFKB-p105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 87-103% 95-106% 87-101%
EDTA plasma(n=5) 85-97% 93-101% 85-98% 90-101%
Heparin plasma(n=5) 79-91% 89-103% 82-95% 82-97%