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Human F-C(Free Carnitine) ELISA Kit
ELK9965
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48 T $320.00
96 T $458.00
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Overview

Product name: Human F-C(Free Carnitine) ELISA Kit
Reactivity: Human
Alternative Names: free Carnitine
Assay Type: Sandwich
Sensitivity: 2.31 nmol/mL
Standard: 400 nmol/mL
Detection Range: 6.25-400 nmol/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (nmol/mL) OD Corrected OD
400.00 2.172 2.089
200.00 1.577 1.494
100.00 1.139 1.056
50.00 0.826 0.743
25.00 0.532 0.449
12.50 0.385 0.302
6.25 0.187 0.104
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F-C and the recovery rates were calculated by comparing the measured value to the expected amount of F-C in samples.
Matrix Recovery range Average
serum(n=5) 82-98% 90%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 82-94% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 86-95% 92-103% 81-90%
EDTA plasma(n=5) 81-93% 78-91% 87-98% 95-107%
Heparin plasma(n=5) 88-103% 86-97% 87-102% 89-101%