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Rabbit F1+2(Prothrombin Fragment 1+2) ELISA Kit
ELK9697
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48 T $320.00
96 T $458.00
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Overview

Product name: Rabbit F1+2(Prothrombin Fragment 1+2) ELISA Kit
Reactivity: Rabbit
Alternative Names: F1+2
Assay Type: Sandwich
Sensitivity: 1.39 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Hematology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.135 2.054
100.00 1.608 1.527
50.00 1.196 1.115
25.00 0.875 0.794
12.50 0.527 0.446
6.25 0.387 0.306
3.13 0.156 0.075
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.
Matrix Recovery range Average
serum(n=5) 81-97% 89%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-106% 87-101% 82-97% 95-102%
EDTA plasma(n=5) 83-96% 97-103% 88-103% 86-92%
Heparin plasma(n=5) 91-99% 95-102% 79-91% 87-96%