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Human P-Cadherin(Cadherin, Placental) Microsample ELISA Kit
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ELK9588MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Human P-Cadherin(Cadherin, Placental) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CDH3; CDHP; HJMD; PCAD; Cadherin 3; type 1
Assay Type: Sandwich
Sensitivity: 36 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.50-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction;Neuroscience
Uniprot ID: P22223
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 1.934 1.838
2000.00 1.571 1.475
1000.00 1.178 1.082
500.00 0.830 0.734
250.00 0.516 0.420
125.00 0.371 0.275
62.50 0.251 0.155
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant P-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of P-Cadherin in samples.
Matrix Recovery range Average
serum(n=5) 83-113% 98%
EDTA plasma(n=5) 85-98% 92%
Heparin plasma(n=5) 95-105% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-109% 84-99% 83-102% 84-116%
EDTA plasma(n=5) 81-114% 93-107% 85-112% 88-108%
Heparin plasma(n=5) 90-116% 92-111% 80-111% 94-118%

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