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Human N-MID-OT(N-MIDOsteocalcin) ELISA Kit
ELK9587
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human N-MID-OT(N-MIDOsteocalcin) ELISA Kit
Reactivity: Human
Alternative Names: N-MID-OT
Assay Type: Sandwich
Sensitivity: 0.39 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human N-MID-OT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N-MID-OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human N-MID-OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human N-MID-OT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 2.258 2.156
20.00 1.625 1.523
10.00 1.183 1.081
5.00 0.826 0.724
2.50 0.518 0.416
1.25 0.357 0.255
0.63 0.236 0.134
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant N-MID-OT and the recovery rates were calculated by comparing the measured value to the expected amount of N-MID-OT in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 83-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-MID-OT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-97% 88-105% 79-93% 87-98%
EDTA plasma(n=5) 89-96% 79-98% 79-91% 90-99%
Heparin plasma(n=5) 85-96% 87-98% 95-104% 93-102%