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Human sHLA-G(soluble human leucocyte antigen G) ELISA Kit
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ELK9364
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human sHLA-G(soluble human leucocyte antigen G) ELISA Kit
Reactivity: Human
Alternative Names: sHLA-G
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Apoptosis;Tumor immunity;Infection immunity;Immune molecule;Hematology;Reproductive science;Immunodeficiency;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sHLA-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sHLA-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sHLA-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sHLA-G in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.984 1.898
5.00 1.527 1.441
2.50 1.108 1.022
1.25 0.839 0.753
0.63 0.593 0.507
0.32 0.332 0.246
0.16 0.228 0.142
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sHLA-G and the recovery rates were calculated by comparing the measured value to the expected amount of sHLA-G in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 84-99% 91%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sHLA-G and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 89-102% 92-101% 87-102%
EDTA plasma(n=5) 84-98% 91-98% 83-101% 87-98%
Heparin plasma(n=5) 95-102% 89-99% 85-96% 91-101%