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Rat pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
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ELK9247
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Reactivity: Rat
Alternative Names: pMAPT /pTAU
Assay Type: Sandwich
Sensitivity: 12.64 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample type: serum, plasma, other biological fluids
Assay length: 3.5h
Research Area: intracellular signal transduction and neuron's development and viability.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.131 2.044
1000.00 1.708 1.621
500.00 1.273 1.186
250.00 0.806 0.719
125.00 0.567 0.480
62.50 0.357 0.270
31.25 0.235 0.148
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant pMAPT /pTAU and the recovery rates were calculated by comparing the measured value to the expected amount of pMAPT /pTAU in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 83-99% 95%
Heparin plasma(n=5) 84-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pMAPT /pTAU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-99% 95-102% 82-90% 87-98%
EDTA plasma(n=5) 93-101% 91-99% 83-97% 87-98%
Heparin plasma(n=5) 86-95% 81-96% 87-96% 85-97%