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Rat 8-iso-PGF2α(8-isoprostane) ELISA Kit
ELK9175
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat 8-iso-PGF2α(8-isoprostane) ELISA Kit
Reactivity: Rat
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Assay Type: Competitive Inhibition
Sensitivity: 9.746 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.625-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.227
500.00 0.357
250.00 0.557
125.00 0.895
62.50 1.275
31.25 1.589
15.63 1.835
0.00 2.115

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-iso-PGF2α and the recovery rates were calculated by comparing the measured value to the expected amount of 8-iso-PGF2α in samples.
Matrix Recovery range Average
serum(n=5) 82-98% 90%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-iso-PGF2α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 97-106% 89-102% 81-94% 96-105%
EDTA plasma(n=5) 85-97% 93-101% 97-103% 91-99%
Heparin plasma(n=5) 86-97% 90-98% 80-93% 92-105%