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Human sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
ELK9134
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Human sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
Reactivity: Human
Alternative Names: CD354; Triggering Receptor Expressed On Monocytes-1; sTREM1
Assay Type: Sandwich
Sensitivity: 12.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.3-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTREM-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTREM-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTREM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.135 2.031
1000.00 1.597 1.493
500.00 1.185 1.081
250.00 0.963 0.859
125.00 0.492 0.388
62.50 0.355 0.251
31.25 0.208 0.104
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sTREM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of sTREM-1 in samples.
Matrix Recovery range Average
serum(n=5) 79-97% 88%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 82-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTREM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-92% 89-102% 87-98% 80-92%
EDTA plasma(n=5) 98-105% 92-101% 85-105% 92-104%
Heparin plasma(n=5) 82-96% 95-102% 89-97% 95-106%