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Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
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ELK9058
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48 T $320.00
96 T $458.00
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Overview

Product name: Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
Reactivity: Human
Alternative Names: TPO Ab; TPO-Ab
Assay Type: Sandwich
Sensitivity: 1.14 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.013 1.932
100.00 1.700 1.619
50.00 1.240 1.159
25.00 0.796 0.715
12.50 0.517 0.436
6.25 0.321 0.240
3.13 0.175 0.094
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TPO-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TPO-Ab in samples.
Matrix Recovery range Average
serum(n=5) 78-91% 84%
EDTA plasma(n=5) 80-96% 88%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 82-95% 92-105% 78-102%
EDTA plasma(n=5) 83-96% 79-93% 88-97% 81-103%
Heparin plasma(n=5) 89-101% 81-98% 93-102% 78-92%