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Rabbit CR(Calretinin) ELISA Kit
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ELK9056
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rabbit CR(Calretinin) ELISA Kit
Reactivity: Rabbit
Alternative Names: CR; Calretinin; CALB2; CAB29; CAL2; 29 kDa calbindin; calbindin 2; calbindin 2; ; 29kD; calretinin; calbindin D29K
Assay Type: Competitive Inhibition
Sensitivity: 0.74 nmol/mL
Standard: 80 nmol/mL
Detection Range: 1.25-80 nmol/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 2h
Research Area: Tumor immunity;Neuro science
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit CR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (nmol/mL) OD Corrected OD
80.00 0.195
40.00 0.387
20.00 0.651
10.00 0.895
5.00 1.156
2.50 1.596
1.25 1.796
0.00 2.232

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CR and the recovery rates were calculated by comparing the measured value to the expected amount of CR in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 92-105% 98%
Heparin plasma(n=5) 90-105% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 79-91% 81-92% 87-97%
EDTA plasma(n=5) 95-102% 93-101% 81-89% 85-99%
Heparin plasma(n=5) 95-107% 96-105% 86-93% 91-105%