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Human SNAP23(Synaptosomal-associated protein 23) ELISA Kit
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ELK8992
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48 T $320.00
96 T $458.00
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Overview

Product name: Human SNAP23(Synaptosomal-associated protein 23) ELISA Kit
Reactivity: Human
Alternative Names: Synaptosomal-associated protein 23 ; SNAP-23 ; Vesicle-membrane fusion protein SNAP-23 ; SNAP23
Assay Type: Sandwich
Sensitivity: 27.2 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Cancer
Uniprot ID: O00161
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNAP23. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNAP23. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNAP23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNAP23 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 1.954 1.868
2500.00 1.575 1.489
1250.00 1.186 1.100
625.00 0.805 0.719
312.50 0.503 0.417
156.25 0.393 0.307
78.13 0.216 0.130
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SNAP23 and the recovery rates were calculated by comparing the measured value to the expected amount of SNAP23 in samples.
Matrix Recovery range Average
serum(n=5) 79-97% 88%
EDTA plasma(n=5) 83-98% 90%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SNAP23 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 90-99% 95-102% 82-90%
EDTA plasma(n=5) 85-92% 83-96% 93-102% 88-97%
Heparin plasma(n=5) 85-101% 86-98% 96-107% 86-93%