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Cattle LL-37(Antibacterial Protein LL-37) ELISA Kit
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ELK8983
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Cattle LL-37(Antibacterial Protein LL-37) ELISA Kit
Reactivity: Cattle
Alternative Names: CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type: Sandwich
Sensitivity: 0.27 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.291 2.208
25.00 1.727 1.644
12.50 1.282 1.199
6.25 0.895 0.812
3.13 0.553 0.470
1.57 0.387 0.304
0.79 0.256 0.173
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.
Matrix Recovery range Average
serum(n=5) 78-93% 85%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 96-103% 87-98% 95-102%
EDTA plasma(n=5) 88-102% 86-92% 81-93% 89-104%
Heparin plasma(n=5) 89-99% 83-95% 98-104% 96-106%