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Chicken 5-HT(5-Hydroxytryptamine) ELISA Kit
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ELK8948
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Chicken 5-HT(5-Hydroxytryptamine) ELISA Kit
Reactivity: Chicken
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma
Assay Type: Competitive Inhibition
Sensitivity: 3.99 ng/mL
Standard: 900 ng/mL
Detection Range: 14.07-900 ng/mL
Sample type: serum, plasma, tissue homogenates
Assay length: 2h
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
900.00 0.191
450.00 0.411
225.00 0.596
112.50 0.913
56.25 1.112
28.13 1.583
14.07 1.762
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.
Matrix Recovery range Average
serum(n=5) 78-96% 87%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 80-95% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 79-92% 92-101% 82-90%
EDTA plasma(n=5) 85-92% 95-103% 97-105% 93-102%
Heparin plasma(n=5) 88-97% 96-105% 85-93% 89-97%