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Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) Microsample ELISA Kit
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ELK8920MS
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48 T $320.00
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Overview

Product name: Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) Microsample ELISA Kit
Reactivity: Human
Alternative Names: Amyloid Beta Peptide 1-42;
Assay Type: Sandwich
Sensitivity: 1.3 pg/mL
Standard: 300 pg/mL
Detection Range: 4.69-300 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
300.00 2.152 2.067
150.00 1.658 1.573
75.00 1.169 1.084
37.50 0.896 0.811
18.75 0.542 0.457
9.38 0.384 0.299
4.69 0.171 0.086
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of hs Ab1-42 in samples.
Matrix Recovery range Average
serum(n=5) 83-112% 98%
EDTA plasma(n=5) 93-120% 107%
Heparin plasma(n=5) 80-119% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-117% 94-116% 81-111% 92-110%
EDTA plasma(n=5) 95-115% 92-98% 89-110% 83-102%
Heparin plasma(n=5) 89-115% 89-109% 84-114% 89-113%

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