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Human PNX-20(Phoenixin-20) ELISA Kit
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ELK8899
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48 T $412.00
96 T $588.00
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Overview

Product name: Human PNX-20(Phoenixin-20) ELISA Kit
Reactivity: Human
Alternative Names: Small integral membrane protein; Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa; MITRAC7; Phoenixin-14; PNX-14; Phoenixin-20; PNX-20; SMIM20; C4orf52
Assay Type: Sandwich
Sensitivity: 7.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Uniprot ID: Q8N5G0
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNX-20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNX-20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNX-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNX-20 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.266 2.171
500.00 1.642 1.547
250.00 1.196 1.101
125.00 0.821 0.726
62.50 0.585 0.490
31.25 0.325 0.230
15.63 0.241 0.146
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PNX-20 and the recovery rates were calculated by comparing the measured value to the expected amount of PNX-20 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 85-98% 91%
Heparin plasma(n=5) 96-107% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNX-20 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-101% 84-98% 85-93%
EDTA plasma(n=5) 85-99% 98-106% 86-99% 78-95%
Heparin plasma(n=5) 96-101% 85-92% 79-96% 89-101%