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High Sensitivity Human I-PTH(IntactParathormone) ELISA Kit
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ELK8878
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48 T $412.00
96 T $588.00
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Overview

Product name: High Sensitivity Human I-PTH(IntactParathormone) ELISA Kit
Reactivity: Human
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Type: Sandwich
Sensitivity: 0.9 pg/mL
Standard: 200 pg/mL
Detection Range: 3.13-200 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
200.00 2.207 2.111
100.00 1.642 1.546
50.00 1.067 0.971
25.00 0.933 0.837
12.50 0.576 0.480
6.25 0.239 0.143
3.13 0.225 0.129
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 78-90% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 85-93% 97-105% 88-97%
EDTA plasma(n=5) 85-93% 87-96% 79-91% 95-103%
Heparin plasma(n=5) 78-95% 86-92% 89-96% 91-97%