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Rat HPLA(p-hydroxyphenyllactic acid) ELISA Kit
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ELK8872
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: Rat HPLA(p-hydroxyphenyllactic acid) ELISA Kit
Reactivity: Rat
Alternative Names: HPLA
Assay Type: Competitive Inhibition
Sensitivity: 6.62 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.6-1000 ng/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: metabolize
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HPLA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HPLA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HPLA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 0.197
500.00 0.334
250.00 0.611
125.00 0.856
62.50 1.156
31.25 1.583
15.63 1.775
0.00 2.132

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HPLA and the recovery rates were calculated by comparing the measured value to the expected amount of HPLA in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 94-105% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HPLA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 87-96% 79-91% 82-91%
EDTA plasma(n=5) 86-95% 82-93% 97-106% 89-102%
Heparin plasma(n=5) 83-92% 86-97% 81-103% 88-97%