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Rat ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit
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ELK8811
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit
Reactivity: Rat
Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide
Assay Type: Sandwich
Sensitivity: 3.2 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;
Uniprot ID: P13589
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADCYAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADCYAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADCYAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADCYAP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.058 1.964
250.00 1.556 1.462
125.00 1.133 1.039
62.50 0.933 0.839
31.25 0.543 0.449
15.63 0.353 0.259
7.82 0.215 0.121
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ADCYAP1 and the recovery rates were calculated by comparing the measured value to the expected amount of ADCYAP1 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADCYAP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 93-101% 86-97% 90-101%
EDTA plasma(n=5) 91-97% 81-93% 78-91% 87-98%
Heparin plasma(n=5) 86-97% 90-101% 93-101% 87-98%