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Human HLA-DR(Leukocyte Antigen DR) ELISA Kit
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ELK8747
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48 T $320.00
96 T $458.00
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Overview

Product name: Human HLA-DR(Leukocyte Antigen DR) ELISA Kit
Reactivity: Human
Alternative Names: HALDR; Human leukocyte antigen DR
Assay Type: Sandwich
Sensitivity: 12.9 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule
Uniprot ID: P01903
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-DR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-DR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-DR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-DR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.126 2.033
1000.00 1.679 1.586
500.00 1.186 1.093
250.00 0.805 0.712
125.00 0.539 0.446
62.50 0.357 0.264
31.25 0.219 0.126
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HLA-DR and the recovery rates were calculated by comparing the measured value to the expected amount of HLA-DR in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HLA-DR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 82-98% 89-97% 85-93%
EDTA plasma(n=5) 87-102% 85-94% 87-101% 89-99%
Heparin plasma(n=5) 95-104% 85-96% 87-102% 91-99%