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Avian 5-HT(5-Hydroxytryptamine) ELISA Kit
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ELK8694
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Avian 5-HT(5-Hydroxytryptamine) ELISA Kit
Reactivity: Avian
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma
Assay Type: Competitive Inhibition
Sensitivity: 4 ng/mL
Standard: 900 ng/mL
Detection Range: 14.07-900 ng/mL
Sample type: serum and plasma
Assay length: 2h
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
900.00 0.191
450.00 0.479
225.00 0.675
112.50 0.967
56.25 1.157
28.13 1.511
14.07 1.813
0.00 2.211

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Avian 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of Avian 5-HT in samples.
Matrix Recovery range Average
serum(n=5) 94-106% 100%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 77-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Avian 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-95% 88-102% 85-96% 90-99%
EDTA plasma(n=5) 82-96% 95-102% 87-98% 81-97%
Heparin plasma(n=5) 86-93% 91-101% 87-98% 95-104%